Unusual Proteolytic Processing of a δ-Endotoxin from Bacillus thuringiensis Strain Buibui by Larval Midgut-Juice of Anomala cuprea HOPE (Coleoptera: Scarabaeidae)
نویسندگان
چکیده
منابع مشابه
Specificity of Bacillus thuringiensis Delta-Endotoxin.
The insecticidal activity of the delta-endotoxins of 14 Bacillus thuringiensis strains belonging to 12 subspecies was determined against Pieris brassicae, Heliothis virescens, and Spodoptera littoralis. Larvae of P. brassicae were highly susceptible to purified crystals of strains of B. thuringiensis subsp. thuringiensis and B. thuringiensis subsp. morrisoni, whereas H. virescens responded best...
متن کاملIncreased production of Coleoptera-specifi c delta-endotoxin by homologous expression of cry3Aa11 in Bacillus thuringiensis Mm2
Th e cry3Aa11 gene of Bacillus thuringiensis strain Mm2 (B. thuringiensis Mm2) was PCR-amplifi ed and cloned into E. coli/B. thuringiensis shuttle vector pHT315. Th e recombinant plasmid carrying the cry3Aa11 gene was introduced into the parental B. thuringiensis Mm2. Th e toxin production capacities of parental and recombinant strains grown in DSM, containing either 50 mM or 200 mM inorganic p...
متن کاملComparative Proteomic Analysis of Aedes aegypti Larval Midgut after Intoxication with Cry11Aa Toxin from Bacillus thuringiensis
Cry toxins produced by Bacillus thuringiensis bacteria are environmentally safe alternatives to control insect pests. They are pore-forming toxins that specifically affect cell permeability and cellular integrity of insect-midgut cells. In this work we analyzed the defensive response of Aedes aegypti larva to Cry11Aa toxin intoxication by proteomic and functional genomic analyses. Two dimension...
متن کاملBarium and calcium block Bacillus thuringiensis subspecies Kurstaki delta-endotoxin inhibition of potassium current across isolated midgut of larval Manduca sexta.
Ba2+ and Ca2+ prevent and reverse the Btk delta-endotoxin inhibition of the short-circuit current in isolated lepidopteran midgut. These findings support the K+ pump-leak steady-state model for midgut K+ homeostasis and the K+ channel mechanism of Bt toxin action. They provide a new tool with which to study the interactions between Bt toxin and midgut cell membranes.
متن کاملTranscriptome of the Lymantria dispar (Gypsy Moth) Larval Midgut in Response to Infection by Bacillus thuringiensis
Transcriptomic profiles of the serious lepidopteran insect pest Lymantria dispar (gypsy moth) were characterized in the larval midgut in response to infection by Bacillus thuringiensis kurstaki, a biopesticide commonly used for its control. RNA-Seq approaches were used to define a set of 49,613 assembled transcript sequences, of which 838, 1,248 and 3,305 were respectively partitioned into high...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Entomology and Zoology
سال: 1997
ISSN: 0003-6862,1347-605X
DOI: 10.1303/aez.32.533